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| Poster #352 The role of carbonic anhydrase VI (CA6) in bitter taste perception |
| Kamila D. Nixon1, Verenice Ascencio Gutierrez1, Samantha L. Brooker1, Emily Demieri1, Fabrice Neiers2, Jeanne Chaloyard2, Ann-Marie Torregrossa1,3 1Department of Psychology, University at Buffalo, Buffalo, NY, United States 2Laboratoire Interdisciplinaire Carnot de Bourgogne, Dijon, --, France 3Center for Ingestive Behavior Research, University at Buffalo, Buffalo, NY, United States |
Salivary proteins (SPs) appear to play a role in bitter taste perception. Our lab has demonstrated in a taste reactivity (TR) paradigm, that rats infused with salivary proteins while they are receiving a quinine stimulus show fewer aversive responses compared to those infused with quinine and saliva that has no proteins present. However, the specific proteins contributing to changes in taste responding remain unidentified. Carbonic anhydrase VI (CA6), a secretory isozyme that is expressed in salivary glands and secreted into the saliva, has been thought to aid in bitter taste perception. To test the role of CA6 in taste responding we delivered recombinantly produced and purified CA6 to rats in the taste reactivity paradigm with and without quinine. We found that when 1mM quinine was administered with CA6, there were less aversive responses than when administering quinine alone. There were no differences in the amount of ingestive responses when administering quinine with or without CA6. Our data suggest that CA6 may be decreasing the bitterness of the stimulus. These findings are in contrast to findings from KO studies. Car6 KO mice appear to increase acceptance for 3µM quinine (Patrikainen et al., 2014). It is currently unclear if the differences between the studies are due to concentration differences, methodological differences, or if the potential role for CA6 in taste bud health (Henkin et al., 1999; Melis et al., 2013) may have played a role in the KO. |