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| Poster #277 Regenerative Potential of Cannabidiol in Chemically-ablated Olfactory Epithelium |
| Bridger Menlove1,2, Olivia C. Turner2, Chloe Crespi2, Madison Klick2, Franklin Pacheco1, Zachary Arnold1, Debra A. Fadool1, 2, 3 1Program in Neuroscience The Florida State University, Tallahassee, FL, United States 2Department of Biology The Florida State University, Tallahassee, FL, United States 3Institute of Molecular Biophysics The Florida State University, Tallahassee, FL, United States |
Cannabidiol (CBD), a non-psychoactive compound of Cannabis, has been implicated in promoting neurogenesis in the central nervous system. This study investigated whether CBD enhances olfactory sensory neuron (OSN) regeneration and functional recovery following methimazole (MeZ)-induced anosmia. OMPtauGFP male mice were orally administered 100 mg/kg CBD in jam daily for two weeks before a single MeZ injection (75 mg/kg) and throughout the 30-day recovery period. Using the buried-cookie test, MeZ-treated mice exhibited anosmia but there was no main effect of CBD drug on cookie retrieval (2-w mixed factorial ANOVA, p = 0.4096). CBD, however, did increase the main olfactory epithelial thickness at 10 days (CBD = 42.50 + 1.97 μm vs. Control = 36.12 + 2.76 μm; Student’s t-test, p = 0.0411), an effect absent at 30 days (p = 0.1610). There was no significant difference in OSN density during recovery (2-w ANOVA, p = 0.1556) nor changes in Cas3 immunolabeling as a marker for cell death as sampled at day 30 (Student’s t-test, p = 0.1301). We are currently sampling whether there are CBD-induced changes in neurogenesis at an intermediate time point of 15 days post MeZ ablation. Currently our findings suggest a transient effect of CBD on epithelial structure, and no substantial influence on mature OSN lifespan or olfactory recovery based upon timing of functional anosmia. |