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| Poster #160 The Roles of the Transcription Factor SPIB in the Taste Papillae. |
| Abdul Hamid Siddiqui, Salin Raj Palayyan, Sunil K Sukumaran University of Nebraska- Lincoln, Lincoln, NE, United States |
We recently showed that type II taste cells may mediate mucosal immune surveillance. They express Spib, a transcription factor that plays a key role in mucosal immunity in microfold (M) cells. Spib knockout (SpibKO) mice have heightened attraction to sweet and umami tastants and a were dramatically altered taste cell. Recent scRNASeq experiments showed that duct cells of the circumvallate and foliate papillae -associated Von Ebners’ gland might also be involved in mucosal immunity. scRNASeq and histological experiments showed that taste papillae contain a higher density of immune cells compared to neighbouring non-taste tissue. Expression of Spib and other M cell marker genes in taste and duct cells were upregulated upon administration of the M cell specific growth factor RANKL. To dissect the roles of SPIB in taste and duct cells, we developed a Spib conditional knockout (SpibCKO) mouse strain. Tamoxifen-induced Spib ablation in this strain triggered severe inflammation in the taste papillae and lingual epithelium and downregulated the expression of multiple taste marker genes. Bulk RNASeq was used to determine the changes in gene expression in taste buds and Von Ebners’ gland duct cells. Further analysis of changes in taste responses and mucosal immune responses will shed light on the roles of SPIB in taste signaling, taste cell regeneration and mucosal immunity. |