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| Poster #240 Long-term Sugar Exposure Increases the Consumption for Glucose Over Fructose in both C57BL/6J and T1R2+3KO Mice |
| David W. Pittman1, Tatiyana L. Adkins1, Mackenzie L. Clinch1, Riley B. Gettys1, Lindsey A. Schier2 1Wofford College, Spartanburg, SC, United States 2University of Southern California, Los Angeles, CA, United States |
Schier et al. previously showed that a sugar exposure paradigm selectively increased licking to glucose relative to fructose in mice. Pittman et al. then showed that sugar-exposed mice showed significantly higher neural activity in the NST for glucose but not for fructose or any of the other non-sweet taste stimuli. In this study, we assessed microstructural licking patterns during 23 h exposures to three concentrations (0.316, 0.560, 1.1 M) of glucose and fructose each presented once in three sets across an 18-day long-term exposure paradigm in female and male wildtype (WT) and Tas1r2 plus Tas1r3 genetic knockouts (KO). All mice innately preferred 0.316 M glucose to all other glucose and fructose concentrations with fructose having the lowest consumptions. Across the three exposures, WT and KO males showed similar increased consumption of 0.316 and 0.56 M glucose but only KO males increased consumption of 1.1 M glucose. In contrast, KO females showed higher consumption for each glucose concentration than the WT females with consumptions increasing across the three exposures. Fructose was the least consumed; however, WT males consumed more fructose than KO males. In contrast, WT and KO females showed similar consumption patterns for fructose that increased for 0.316 M fructose from the first to the second and third exposures with no change in consumption of 0.56 or 1.1 M fructose across the three exposures. Increases in the number of meals rather than the number of licks within the meals appear to account for the differences in consumption. In summary, there are both genotypic and sex differences in consumption of glucose and fructose across an 18-day exposure paradigm. Additional microstructural analysis of licking patterns will be explored in the poster. |