Presentation Details
Fast Updating Feedback From the Piriform Cortex to the Olfactory Bulb Relays Multimodal Identity and Reward Contingency Signals During Rule-Reversal

Diego E Hernandez Trejo1, Andrei Ciuparu2, Pedro Garcia da Silva3, Cristina M Velazquez Cobos4, Benjamin Rebouillat5, Michael Gross1, Honggoo Chae1, Martin B Davis 1, Raul C Mureşan2, 6, Dinu F Albeanu1, 7.

1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.2Transylvanian Institute of Neuroscience, Cluj-Napoca, Rumania.3Champalimaud Foundation, Lisbon, Portugal.4Oxford University, London, United Kingdom.5Université Paris 8 Vincennes - Saint-Denis, Paris, France.6STAR-UBB Institute, Babeş-Bolyai University, Cluj-Napoca, Rumania.7School for Biological Sciences, CSHL, Cold Spring Harbor, NY, USA

Abstract


Animals adjust their behavior to adapt to relevant environmental changes, but the neural pathways enabling these changes remain unclear. Mice excel in discriminating odorants in complex sensory conditions. However, little is known about (1) how changes in stimulus contingency modify odor representations and (2) how updating odor representations is causally related to behavioral adjustments. The anterior piriform cortex (aPCx) sends dense feedback to the olfactory bulb (OB) and shapes, specifically the activity of mitral cells (MCs), one of the OB output channels. However, the role of aPCx feedback in shaping bulb output according to behavioral needs remains unclear. To investigate the role of aPCx feedback in supporting flexible behaviors, we designed a novel Go/No-Go task with rule reversal guided by olfactory and auditory cues. Within the same session, stimulus-reward contingencies were reversed across blocks of trials. In parallel, we monitored the aPCx-to-OB feedback (GCaMP) using multiphoton microscopy. The aPCx feedback activity triggered by the task cues preceded the behavioral reporting (licking) and mirrored the reversals in stimulus-reward contingency throughout each session. Within seconds of each rule reversal, we observed the re-shaping of individual bouton responses to the same sensory cue in tight correlation with the behavioral output switch. Optogenetic perturbation of the aPCx feedback within the OB (Jaws) disrupted the behavioral performance. Our results indicate that the aPCx-to-OB feedback multiplexes stimulus identity and reward contingency signals and is rapidly re-formatted according to changes in the behavioral output. In ongoing experiments, we analyze the interplay between OB feedforward and aPCx feedback signals supporting behavioral flexibility.

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