Presentation Details
| Asymmetric Histone Inheritance Regulates Olfactory Stem Cell Fates During Regeneration Binbin Ma1, 2, Guanghui Yang1, 2, Jonathan Yao2, Charles Wu2, Jean P Vega2, Gabriel Manske3, Saher S Hammoud3, Satrajit Sinha4, Abhyudai Singh5, Haiqing Zhao2, Xin Chen1, 2. 1Howard Hughes Medical Institute, Baltimore, MD, USA.2Johns Hopkins University, Baltimore, MD, USA.3University of Michigan, Ann Arbor, MI, USA.4SUNY at Buffalo, Buffalo, NY, USA.5University of Delaware, Newark, DE, USA |
Abstract
The olfactory epithelium (OE) possesses an adult stem cell population, the horizontal basal cells (HBCs), to permit lifelong tissue regeneration. Here we show that HBCs exhibit asymmetric inheritance of histone H4 but not H2A-H2B during OE regeneration in mice. Primary HBC cultures further revealed asymmetric histone inheritance for H3 and H3.3. Upon mitotic exit, asymmetric histone inheritance correlates with asynchronous transcription re-initiation and differential enrichment of p63, a key transcription factor for HBC cell fate. Disruption of asymmetric histone inheritance abolished these asymmetric cellular features and attenuated OE regeneration and smell behavior recovery. Single-cell RNA sequencing of paired HBC daughters in culture further supported asymmetric multilineage cell fate priming. Together, these findings reveal asymmetric histone inheritance in a mammalian adult stem cell lineage and highlight its biological significance in neural tissue regeneration and animal behavior.
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No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.
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