Presentation Details
| Evidence for Modulation of the Sour Taste Receptor OTOP1 by Compounds Associated with Licorice Aftertaste sabrina Corazza, Manuel Arcangeletti, Irene Riva, Menella Valotta, Katja Blasi, Marcel Winnig, Jean-Francois Rolland. Axxam S.pA, Bresso, Milano, Italy, Italy |
Abstract
OTOP1 is a proton-selective ion channel expressed in taste receptor cells and has been identified as a critical molecular sensor for sour taste (Tu et al., 2018; Sladkov & Kolesnikov, 2024). In addition, OTOP1 has recently been reported to act as a sensor for ammonium chloride (NHâ‚„Cl), a tastant described as bitter, salty, and mildly sour, which is a key component of salty licorice (Zhang et al., 2023). In this study, we developed a reliable high throughput automated electrophysiological assay to investigate OTOP1 function and modulation. Human OTOP1 was transiently expressed in CHO cells using MaxCyte electroporation, and recording conditions were optimized on the SyncroPatch 384 platform to achieve stable and reproducible measurements of proton-mediated currents. We then tested a panel of sweet-tasting compounds, selected based on their association with a licorice-like aftertaste. Several compounds exhibited inhibitory effects on OTOP1-mediated proton currents at the SyncroPatch 384. To validate these findings, three representative compounds (one strongly associated with licorice aftertaste, one showing weaker activity and one negative control) were further examined using the golden standard method for electrophysiology i.e. manual patch-clamp confirming the effects observed in the automated assay. Together, these findings establish a robust assay for studying OTOP1 pharmacology and provide evidence that sweetener-associated compounds can modulate a key taste receptor involved in sour and ammonium taste, offering new insight into molecular mechanisms underlying complex taste perceptions.
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No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.
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