Presentation Details
| Immature olfactory sensory neurons provide complementary input in the healthy olfactory system Jordan D.Gregory1, 2, Ryan S.Herzog1, 2, Kendall A.Curtis1, Michael I.Marar1, Claire E.J.Cheetham1. 1Department of Neurobiology, University of Pittsburgh, Pittsburgh, PA, USA.2Center for Neuroscience at the University of Pittsburgh, Pittsburgh, PA, USA |
Abstract
Adult neurogenesis of olfactory sensory neurons (OSNs) in the rodent olfactory system provides the unique opportunity to understand how new neurons functionally integrate into existing circuitry and contribute to behaviors. Immature OSNs express odorant receptors (ORs), form dendritic knobs with short cilia, and project their axons into the olfactory bulb (OB) where they form functional synapses. Furthermore, immature OSNs respond selectively to odorants and exhibit graded responses in a higher concentration range than mature OSNs, suggesting that they provide a distinct odor input stream. Finally, in mice that lack mature OSNs, sensory input from exclusively immature OSNs is sufficient to mediate odor detection and discrimination behavior. What remains unknown, however, is how these immature OSNs contribute to odor-guided behavior in the healthy, intact olfactory system. Here we show, using male and female mice, that chemogenetically silencing immature OSNs impairs odor detection ability without affecting their odor discrimination ability. Furthermore, silencing immature OSNs reduced odor-evoked responses in OB neurons based on immediate early gene expression. Finally, silencing immature OSNs reduced the amplitude of odor-evoked dendritic calcium responses in OB output neurons in vivo without affecting odor selectivity. Together, these findings suggest that immature OSNs provide distinct odor input that complements mature OSN input to contribute to odor-guided behaviors in the healthy, intact olfactory system.
No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.
No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.
Content Locked. Log into a registered attendee account to access this presentation.