Presentation Details
| Mapping Fos-immunoreactive neurons activated by intra-oral infusion of quinine, sucrose or water throughout the brain of B6 mice Michael S King1, Lianyi Lu2, Max L Fletcher2, John D Jr Boughter2. 1Stetson University, DeLand, FL, USA.2The University of Tennessee Health Science Center, Memphis, TN, USA |
Abstract
Fos immunohistochemistry was used to identify neurons throughout the B6 mouse brain activated by intra-oral (IO) infusion of 3.0 mM quinine hydrochloride (Q), 1.0 M sucrose (S) or filtered water (W). Fos-immunoreactive (Fos-IR) neurons were found in each nucleus and subarea examined following each treatment with a few differences in the number of labeled neurons among treatments. Specifically, IO infusion of Q and S elicited more Fos-IR neurons than W in the central medial (CM) and dorsomedial (DM) subareas of the parabrachial nucleus (PBN) and the central medial (CeM) amygdala (ps<0.05). Infusion of Q led to more Fos-IR neurons than W in the central lateral (CL) PBN and the parvocellular reticular formation (PCRT, ps<0.05). The only area where IO infusion of Q and S elicited a different number of Fos-IR neurons was the PCRT which responded more to Q (p<0.05). Therefore, IO infusion of Q and S did not activate a different number of neurons in almost all taste-related brain areas. However, a cluster analysis of the number of Fos-IR neurons in all 29 nuclei and subareas examined revealed that populations of neurons distributed among these brain regions respond best to Q, S or both Q and S. Specifically, the Q-best cluster tended to include more posterior structures like the nucleus of the solitary tract, RT and most of the PBN. The S-best cluster included more anterior structures like the bed nucleus of the stria terminalis, nucleus accumbens and orbitofrontal cortex. And, the cluster of areas that responded better to Q and S than W included the amygdala, gustatory and piriform cortices and a few PBN subareas. Therefore, the data suggest that collections of neurons among taste-responsive brain areas are important for distinguishing Q and S from water as well as identifying the specific tastant.
No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.
No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.
Content Locked. Log into a registered attendee account to access this presentation.