Presentation Details
| Compounded Effects of E-Cigarette Aerosol Components on Glomerulus Size and Sniffing Patterns in Mice" Sean O'Sullivan, Farhan Augustine, Virginia Murray, Kafui Ameko, Iskandar Nassar, Silvi Shah, Setutsi Abdallah, Tatsuya Ogura, Weihong Lin. Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore, MD, USA |
Abstract
Electronic cigarette (e-cigarette) usage is a growing activity among teens and young adults, with millions engaging in daily use. Aerosols produced by the combustion of e-cigarette liquids release flavorants along with harmful chemicals such as formaldehyde, toluene, other volatile hydrocarbons, and metals which can interact with the olfactory system through first and secondhand exposure. We previously observed aerosol exposure from e-cigarette liquids (e-liquids) with spiked heavy metals, simulating long-term vaping, reduced olfactory epithelium thickness, numbers of mature olfactory neurons, and glomerulus sizes. Following this, we investigated what the flavor components of e-liquid affect the specific glomerulus which senses a particular odor and how sniffing behavior of said odors was altered. Groups of mice were exposed twice daily for 8 weeks to simulate subchronic e-cigarette use, during which we assessed sniffing behaviors of e-liquid components at 0/4/8 weeks, following with harvesting and imaging olfactory bulbs from the mice. Exposure conditions consisted of air, propylene glycol/vegetable glycerin (PG/VG), PG/VG based e-liquid containing nicotine and OR151/M71 agonists, and e-liquid spiked with heavy metals. We observed that repeated exposure to e-liquid with the agonists induced increased size of the cognate glomerulus, but spiking the e-liquid with metals decreased size. Furthermore, both e-liquid exposure groups showed markedly less interaction times with e-liquid components. These findings suggest that repeated e-cigarette exposure reduces sniffing behavior and corresponding ligand glomerulus size, possibly due to toxic effects of the aerosol and/or desensitization to the odorants in the aerosol.
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No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.
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