Presentation Details
| The many ways to be bitter Thirada Boonrawd, Syed Adnan Uddin, Hojoon Lee. Northwestern University, Evanston, IL, USA |
Abstract
The sense of taste allows animals to distinguish between nutritious foods and poisonous compounds in their diet. Taste receptor cells (TRCs) within taste buds express dedicated receptors to detect the five taste qualities: sweet, umami, bitter, salty, and sour. In mice, the Tas1r2 and Tas1r3 dimer forms the receptor for sugars. The Tas1r1 and Tas1r3 dimer forms the receptor for amino acids. Meanwhile, there are 35 known bitter receptors (Tas2rs), which are important for detecting numerous noxious compounds in the diet. Previous studies have only used dual color in situ hybridization to investigate the expression patterns of Tas1rs and Tas2rs. Therefore, the co-expression patterns of groups of Tas2rs remains unknown. To address this knowledge gap, we tracked co-expression of multiple Tas2rs and Tas1rs in the TRCs of the mouse circumvallate papilla and fungiform papilla using RNAscope, a multiplexed mRNA FISH method. I will present ongoing work which suggests that most Tas2rs often co-express together, and are usually not co-expressed with Tas1r2. However, we observe that some Tas1rs and Tas2rs are more broadly expressed than previously thought.
No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.
No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.