Presentation Details
| Discrimination of viral and non-viral cells through volatile organic compound profiling of culture-derived headspace Samantha Hagerty1, Michelle Aono1, Adam Rivers3, Melissa Singletary1, 2. 1Department of Anatomy, Physiology, and Pharmacology, Auburn University, Auburn, AL, USA.2Canine Performance Sciences, Auburn University, Auburn, AL, USA.3United States Department of Agriculture, Agriculture Research Service, Gainesville, FL, USA |
Abstract
The highly advanced canine olfactory system is utilized across fields of odor detection, with recently emerging applications in biological threats and disease surveillance. Selection of training materials is critical for optimal discriminatory performance, where target odors must be representative of the true material and distractor odors must be contextually relevant. Due to the metabolic complexity and unknown odor profiles of most biomaterials, it is challenging to include distinct yet operationally relevant distractors. To understand odor-based discrimination and optimize the application of non-invasive chemosensory detection of infectious agents, we chemically characterized the volatile organic compound (VOC) profiles of two separate cell culture models (Channel Catfish Oocytes-CCO and Madin-Darby Bovine Kidney-MDBK) under eustress and stress conditions through induction of viral infection, thermal stress and/or cellular crowding. Viral targets included Channel Catfish Virus (CCV) and Bovine Viral Diarrhea Virus (BVDV). The viral distractor included Bovine Herpes Virus (BoHV) and non-viral groups included cell-free growth media, healthy cells and/or or alternatively stressed cells through thermal or overcrowding conditions. Real-time headspace analysis using Proton Transfer Reaction-Mass Spectrometry (PTR-MS) demonstrated distinguishable odor signatures for each viral model compared to non-infected controls, as well as shared features across different forms of physiological stress. These results contribute to understanding the volatile signature of viral cell culture models of interest while highlighting the importance of intentional distractor odor selection in canine training to meet the challenge and complexity of biological targets.
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No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.
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