Presentation Details
| Investigating axonal translation at oral sensory nerve terminals reveals a novel role for FGF13 in taste bud innervation and maintenance Debarghya Dutta Banik, Brian Pierchala. Department of Anatomy, Cell Biology & Physiology, Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, IN, USA |
Abstract
Taste buds are comprised of a heterogeneous population of taste receptor cells (TRCs), which are neuroepithelial in nature. TRCs die off every 2-4 weeks and are replaced by new cells. As a result, the gustatory neurons innervating these TRCs undergo constant cycles of innervation, denervation, and re-innervation. This remodeling process relies on a precise balance between signaling cues from TRCs and the surrounding lingual epithelium, as well as on the reciprocal responses of gustatory neurons. Local translation of axon-localized mRNAs is known to be critical for axon regeneration and synaptogenesis. Despite the importance of axonal translation in neuronal function, no studies have identified axonal transcripts in a physiologically and rapidly remodeling neuronal system, such as the gustatory system. To identify genes translated axonally at the taste synapse, I used an unbiased ribosomal profiling method utilizing the Phox2bCre; RiboTag mouse model. To profile active translation and changes in gene expression under physiological conditions, ribosomes and associated mRNAs were isolated selectively from the axon terminals of Phox2b+ neurons innervating the lingual epithelium. HA-tagged, ribosome-associated RNAs were purified from lingual epithelium containing Fungiform and Circumvallate papillae. RNA-sequencing data reveal that Fibroblast Growth Factor 13 (Fgf13) is highly enriched at chemosensory neuron terminals and largely absent from cell bodies. Loss of FGF13 in taste neurons leads to a severe loss of taste bud innervation and taste buds in both the CV and Fungiform papillae. These results suggest a novel role of axonal translation and axonally translated genes in the maintenance of taste bud innervation and taste buds.
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