Presentation Details
| Developing von Ebner’s Glands in Embryos Are the Main Source of Sox10⁺ Progenitors for Taste Buds in Postnatal Mice Md Mamunur Rashid, Yufei Huan, Hong-Xiang Liu. Regenerative Bioscience Center, Department of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, Athens, GA, USA |
Abstract
We have recently found that von Ebner’s glands (vEGs) contain Sox10⁺ progenitors for circumvallate papilla (CvP) taste buds using Sox10-Cre for lineage tracing and single-cell RNA sequencing. Sox10-Cre-traced cells comprise ~40% taste bud cells in adult mice. However, tamoxifen treatment of Sox10-CreERT2/RFP mice after birth results in far fewer traced taste bud cells than Sox10-Cre. Questions remain whether Sox10+ vEG cells can differentiate to taste cells in vitro, and when Sox10+ taste bud progenitors in vEGs are generated. Using taste organoid cultures of CvP/vEG epithelial cells from Sox10-Cre/RFP mice, we generated organoids that contain all three types (I-III) of differentiated taste cells, and detected Sox10-Cre/RFP⁺ taste cells in organoids. To define the developmental timing of Sox10⁺ vEG progenitor contribution in vivo, we performed in situ hybridization and immunohistochemistry for identifying Sox10+ vEG cells at various stages. We found that Sox10 is expressed in the emerging vEG at E16.5, and from then on in the developing and developed vEGs. Moreover, Sox10-CreERT2/RFP-traced cells with tamoxifen during late embryogenesis (E15.5–E18.5) were initially confined to vEGs and surrounding tissues but absent in early taste buds at P1. However, embryonically traced cells were populated in taste buds in postnatal mice and progressively more abundant with age. In contrast, only sparse RFP⁺ cells were detected within taste buds when tamoxifen was given to 8-week-old Sox10-CreERT2/RFP mice. Together, these findings demonstrate that Sox10⁺ taste bud progenitors in vEGs are produced mainly at embryonic stages, whereas Sox10⁺ progenitors in adult vEGs contribute to taste buds to a limited extent. Keywords: Progenitor, von Ebner’s glands, Sox10, Circumvallate papilla, Taste bud.
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