Presentation Details
| Odor Stimulation in the Mouse Olfactory Epithelium Promotes Transit Amplification Within a Subset of Neuronal Lineages Alyssa Granley, Madeline Smith, Kawsar Hossain, Stephen Santoro. Department of Pediatrics, Section of Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA |
Abstract
In mice, olfactory sensory neuron progenitors undergo a variable number of cell divisions before differentiating and selecting for expression 1 out of ~1,200 odorant receptors, which define the neuron’s subtype. The process of olfactory sensory neurogenesis has long been assumed to be purely stochastic with respect to subtype. In apparent conflict with this, our lab has found that the birthrates of specific subtypes are selectively increased following exposure to discrete odors that stimulate those subtypes. The mechanism by which this process occurs is unknown. We hypothesize that neural progenitors can be predisposed towards specific subtype fates and can be amplified via cell division due to signals from odor-stimulated neurons, accelerating the birthrates of those subtypes. If this is true, we predicted that odor deprivation should selectively reduce the number of cell divisions observed within a subset of neural lineages. To test this, we first assessed the effects of unilateral naris occlusion on the size distribution of lineages using a Confetti x Kit-CreERT2 mouse model to induce the expression of 1 of 4 fluorescent reporters in neural progenitors and their progeny. As predicted, we observed that odor deprivation decreased the proportion of lineages with large numbers of neurons but did not change the total number of lineages. We next assessed the effects of naris occlusion on how long progenitors undergo transit amplification using a dual thymidine analogue approach. We observed that odor deprivation reduced the proportion of progenitors that continued to divide for at least 48hrs. Our findings support a model where odor-derived signals cause progenitors with pre-disposed subtype fates to undergo more rounds of division, leading to selective increases in the birthrates of these subtypes.
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