Presentation Details
| Activity dependent regulation of gene expression and chromatin structure in mouse olfactory sensory neurons Joshua Danoff, Kevin Monahan. Rutgers University, Piscataway, NJ, USA |
Abstract
Olfactory sensory neurons (OSNs) calibrate to odor environments by dampening their response to abundant odorants and heightening their response to rare odorants. This calibration requires transcriptional changes in genes that impact neuronal excitability, ultimately tuning the activity of each OSN to its environment. Using ATAC-seq and Micro-C, we investigate how chromatin structure differs in highly active and inactive neurons. First, we find abundant differential chromatin accessibility among OSNs that are highly active compared to those that are not active. Activity-open peaks are enriched for known transcription factors in OSNs, including Lhx2 and Ebf, and the neuronal activity-dependent transcription factor Nfat. Gene ontology analysis indicates that activity-open peaks are associated with genes involved in synaptic transmission and olfactory perception. We then use Micro-C to compare 3D genome structures in highly active and inactive neurons, focusing on enhancer-promoter interactions at activity dependent genes. Using these approaches, we have identified how chromatin organization is modified by neuronal activity and enables expression of activity dependent genes, an essential process for olfactory sensory neurons to calibrate excitability to their environment.
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No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.